Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. More specifically, all referenced documents are incorporated by reference to the same extent as if each individual document was specifically and individually indicated to be incorporated by reference. The government has certain rights in the invention. Precise genome targeting technologies are needed to enable systematic reverse engineering of causal genetic variations by allowing selective perturbation of individual genetic elements, as well as to advance synthetic biology, biotechnological, and medical applications.
The residents of Avtar Nagar, Hargobind Nagar and the slum area on the Mudki road in Baghapurana are a harried lot, especially in the wake of three diarrhoea deaths in the neighbouring Mari Mustafa village last week. Residents of these areas were also consuming hand pump water like the villagers of diarrhoea-hit Mari Mustafa.
These hand pumps were installed on 60 to 70 feet deep bores, while it should be over feet deep for getting clean water. The hand pumps too were installed near their toilets and there were no proper arrangements for sewage disposal.
Tipal dam year-old boy had died after showing diarrhoea-like symptoms in Hargobind Nagar a few weeks back.
Bikar Singh, the father of the deceased, told The Tribune that they had been drinking this water for years and nothing was done to lay pipelines in their locality.
The NAC had done little to maintain cleanliness in the locality and it seemed to be facing neglect all fronts.
There had been a proposal in the past for sewage Tipal dam, but it remained on paper only. Open sewers serve as a breeding ground for mosquitoes, and heaps of garbage could be seen inviting stray cattle. The absence of streetlights makes it nearly impossible for elderly to move out at night.
The slum area on the Mudki road, which is under NAC limits since its inception inis no better, with people bathing, washing clothes and drinking water at a single hand pump, which is surrounded by sewage, garbage heap and cow dung.
Surprisingly, this road leads to the local Civil Hospital and the tehsil complex and the government officials like the SMO, the SDM and the CMO pass through the road, but nobody has noticed what might be a source of various diseases.
Ruparam, a resident of the area, said that they had told the NAC staff to lift the garbage, but they were demanding Rs for the job. He said that they themselves had lifted the garbage on rehris many a time.
Tribune News Service Bathinda, August 5 Watch out while lending your mobile phone to friends lest its chip gets stolen or the programme gets tampered with. This is what has happened to a local resident, Mr Pankaj Goyal, who lent his Samsung mobile phone activated on a Reliance chip to his friend Pali few months ago.
Pali, kept on using his phone for weeks together. Mr Goyal had no idea that both the phones were working on same number. Mr Goyal came to know about this fact when the outgoing and incoming calls on his phone were stopped due to some reasons. When he received the printout of the calls made and received by him, he was shocked to find that he had been charged for calls, he neither received nor made.
Mr Goyal took up the matter with Pali saying that he had been receiving inflated bills and ever since he lent his phone to him and the incoming and outgoing calls were also stopped on his mobile phone, which was a post paid connection.
Mr Goyal also lodged a complaint with the police. Mr Shiv Kumar, SP Hwho conducted the inquiry said that after finding Pali and another person guilty in this case, which was first of its kind in the district, a case under Sectionsand 34 of IPC had been registered. He added that no arrest had been made in this connection so far.Search the history of over billion web pages on the Internet.
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